yvcK [2017-8-11 16:0:3]

essential for growth under gluconeogenetic conditions, control of cell shape, required for correct localization of PBP1

Locus: BSU34760

Isoelectric point: 5.41

Molecular weight: 34.53 kDa

Protein length: 317 aa

Gene length: 951 bp

Function: localization of PBP1

Product: UDP-sugar binding cell shape determinant

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall synthesis → Biosynthesis of peptidoglycan
• Cellular processes → Cell envelope and cell division → Cell shape
• Metabolism → Additional metabolic pathways → Biosynthesis of cell wall components → Biosynthesis of peptidoglycan
• Groups of genes → Efp-dependent proteins
• Groups of genes → Phosphoproteins → Phosphorylation on a Thr residue

Gene

Coordinates: 3,570,546 → 3,571,499

Phenotypes of a mutant

• unable to grow with gluconeogenic substrates as single carbon source PubMed
• filamentous or L-shape-like aberrant morphologies (suppressed by Mg²⁺) PubMed, this is supressed by overexpression of MreB or by deletion of PBP1 PubMed

The protein

Protein family

• UPF0052 family (according to Swiss-Prot)

Domains

• phosphorylated on Thr-304 by PrkC, dephosphorylated by PrpC PubMed

Effectors of protein activity

• binds UDP-sugars (UDP-Glc, UDP-GlcNAc) PubMed

Structure

• 2O2Z (the protein from B. halodurans, 63% identity, 86% similarity)

Localization

• localized as a helical-like pattern PubMed

Expression and Regulation

Operons

• Genes: nahA-yvcJ-glmR-whiA-crh-yvcN
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, in CcpA regulon

  Regulation

  • constitutive expression at both protein and RNA levels PubMed

  Additional information

  • A ncRNA is predicted between 'yvcI' and 'trxB' PubMed

Additional information

• translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

Mutant

• MGNA-B640 (yvcK::erm), available at the NBRP B. subtilis, Japan

Expression vector

• pGP736 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab

Labs working on this gene/protein

• Anne Galinier, University of Marseille, France

References

Reviews

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Original publications

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